Effect of Xiaoning liquid on gut microbiota in asthmatic mice by 16S rDNA high-throughput sequencing

To investigate the effects of Xiaoning liquid on gut microbiota in mouse during asthma. A total 60 mice were randomly and averagely assigned to healthy control group, budesonide group. The later three groups used establish an Ovalbumin (OVA) asthma model. intestinal bacterial communities compared among using 16S rRNA gene amplification. Analyzing structure with OTU analysis, Shannon-Wiener, PCA, PCOA, etc. rDNA high- throughput sequencing. abundance diversity asthmatic increased, most obviously Bacteroidetes Firmicutes levels increased all mice. level Bacteroides obviously, making a useful marker changes Proteobacterium, Deferribacteraceae Mucispirillum dropped significantly can reduce species numbers pathogenic bacteria restored microecology has positive effect function microbiota.